Introduction of antibody into viable cells using electroporation
- 1 January 1991
- Vol. 12 (1) , 64-67
- https://doi.org/10.1002/cyto.990120109
Abstract
Conditions for labelling an intracellular antigen, p21ras, using electroporation to introduce a fluorescent antibody, are described. Following labelling, cells were evaluated for p21ras associated fluorescence by flow cytometry. Electroporation, sorting, and cell handling parameters were varied to determine optimal conditions for cell viability. Cells were best held in serum containing growth medium both before and after electroporation, while antibody introduction during the electroporation phase was most efficient when carried out in a balanced saline solution. For maximum efficiency of antibody internalization, the antibody needed to be present during electroporation, and medium needed to be replaced several times in the first few hours after electroporation to ensure good cell survival.Keywords
This publication has 5 references indexed in Scilit:
- Isolation of viable tumor cells following introduction of labelled antibody to an intracellular oncogene product using electroporationJournal of Immunological Methods, 1989
- Transfer of monoclonal antibodies into mammalian cells by electroporationJournal of Biological Chemistry, 1989
- Human cancer and cellular oncogenesBiochemical Journal, 1987
- Electroporation for the efficient transfection of mammalian cells with DNANucleic Acids Research, 1987
- Influence of migratory blood cells on the attachment of tumor cells to vascular endotheliumInternational Journal of Cancer, 1984