RAPID INDUCTION OF CARCINOMAS AND GAMMA-GLUTAMYL-TRANSFERASE TRANSPEPTIDASE-RICH CLONES IN N-METHYL-N-BENZYLNITROSAMINE-TREATED HAMSTER BUCCAL POUCH

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  • 1 January 1985
    • journal article
    • research article
    • Vol. 74  (2) , 437-445
Abstract
Topical application of N-methyl-N-benzylnitrosamine [(MBN) CAS: 937-40-6] to Syrian hamster buccal pouch epithelia resulted within 4 days in the induction of epithelial cell foci with histochemical .gamma.-glutamyl transpeptidase (GGTase) activity. Approximately 94% of the foci consisted of individual cells or nests of 8 cells or less, as visualized in wholemounts of GGTAse-stained pouch epithelia. During a regimen of twice weekly MBN applications, the GGTase-stained foci increased in size to a maximum diameter of .apprx. 0.4 mm by the 3rd wk. In histologic sections, the enlarging foci were similar morphologically to those of the adjacent GGTase-negative epithelium. Groups of animals treated with MBN for 2, 4 and 8 wk developed a buccal pouch tumor incidence of 43, 86 and 100%, respectively, within 39 wk or less. Of the 138 papillomas and carcinomas examined, 11 contained patchy GGTase activity.

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