Mutation and Selection of Lactobacillus plantarum Strains That Do Not Produce Carbon Dioxide from Malate

Abstract
A differential medium was developed to distinguish between malate-decarboxylating (MDC + ) and -non-decarboxylating (MDC ) strains of Lactobacillus plantarum. MDC strains produced a visible acid reaction in the medium, whereas MDC + strains did not. Use of the medium allowed for rapid screening and isolation of mutagenized cells that had lost the ability to produce CO 2 from malate.

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