Detection of Antigen-Specific Reaginic Antibody in vitro using Human Skin

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Abstract
Human skin removed at operation was used for detection of reaginic activity (antigen-specific IgE) in the serum of allergic patients. Human skin was sliced using a hand microtome. Skin slices were sensitized by reaginic serum from allergic patients, and measurement of the histamine released from sensitized skin slices by specific antigen was carried out by bioassay. Serum from 12 out of 14 patients allergic to grass pollen gave significant antigen-specific histamine release. The sensitivity of this method could be increased by use of ethylenediaminetetraacetic acid during passive sensitization. Storage of the skin for 24 h did not impair its capacity to undergo passive sensitization. This method, which is essentially an in vitro model of Prausnitz-Küstner reaction, should be useful for in vitro diagnosis of immediate type (IgE-mediated) hypersensitivity in man.

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