South African tobaccos. I.—Preliminary identification of amino‐acids and other constituents

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Abstract
Methods of preparing tobacco‐leaf extracts for paper chromatography are described. Preliminary work has been carried out on the free amino‐acids of three varieties of tobacco grown in South Africa, and normally used for flue curing. The following amino‐acids have been detected in tobacco leaf: aspartic acid, asparagine, glutamic acid, glutamine, serine, threonine, alanine, tyrosine, proline, valine, γ‐aminobutyric acid, leucine, isoleucine, phenylalanine, tryptophan, β‐alanine, histidine and lysine. Several substances, as yet unidentified, which give a ninhydrin reaction are also present. The paper‐chromatographic method also appears promising for the study of sugars and polyphenols in tobacco leaf.
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