A Virus-inactivating System from Tobacco Leaves

Publisher Website
Google Scholar
Abstract
The ratio of infectlvity to the amount of antigen contained in purified preparations of Rothamsted tobacco necrosis virus (RTNV) has been found to vary with the treatment of the leaf extracts. A virus-inactivating system has been detected in freshly expressed sap, from which it can be concentrated by ultra-cent rifugation. If centrifugation is delayed the inactivating system is destroyed and consequently less appears in the final virus preparation. The methods are described for the preparation of stable purified virus and of concentrates of the inactivating material. The nature of this substance is unknown but it has the same effect as some aldehydes and derivatives of ascorbic acid.