Molecular cloning and sequencing of cDNAs encoding the entire rat fatty acid synthase.
- 1 May 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (9) , 3114-3118
- https://doi.org/10.1073/pnas.86.9.3114
Abstract
Overlapping cloned cDNAs representing the entire sequence of the rat fatty acid synthase mRNA have been isolated from a cDNA library and sequenced. Authenticity of the cDNA clones was supported by hybridization to fatty acid synthase mRNA and by amino-terminal sequencing of 39 fatty acid synthase CNBr fragments. The full-length fatty acid synthase mRNA is 9156 nucleotides long and includes an 84-nucleotide 5'' noncoding region, a 7515-nucleotide coding sequence, and a 1537-nucleotide 3'' noncoding region; a second mRNA species containing a shortened 3'' noncoding sequence is also transcribed in the rat. The encoded fatty acid synthase subunit contains 2505 amino acids and has a molecular weight of 272,340. Active sites and substrate binding sites were located within the sequence, thus establishing the order of domains on the multifunctional animal fatty acid synthase as condensing enzyme-transferase-dehydrase-enoyl reductase-ketoreductase-acyl carrier protein-thioesterase.This publication has 24 references indexed in Scilit:
- Characterization of a genomic and cDNA clone coding for the thioesterase domain and 3' noncoding region of the chicken liver fatty acid synthase geneBiochemistry, 1988
- Complete amino acid sequence of the medium-chain S-acyl fatty acid synthetase thio ester hydrolase from rat mammary glandBiochemistry, 1987
- Specific modification of the condensation domain of fatty acid synthase and the determination of the primary structure of the modified active site peptidesArchives of Biochemistry and Biophysics, 1984
- A simple and very efficient method for generating cDNA librariesGene, 1983
- Amino acid sequence around the active serine in the acyl transferase domain of rabbit mammary fatty acid synthaseFEBS Letters, 1983
- Sequence of a tryptic peptide from the NADPH binding site of the enoyl reductase domain of fatty acid synthaseArchives of Biochemistry and Biophysics, 1983
- Subunit structure of the mammalian fatty acid synthetase: Further evidence for a homodimerArchives of Biochemistry and Biophysics, 1979
- Specificity and site of action of a mammary gland thioesterase which releases acyl moieties from thioester linkage to the fatty acid synthetaseArchives of Biochemistry and Biophysics, 1979
- Studies on the immunological cross-reactivity and physical properties of fatty acid synthetasesArchives of Biochemistry and Biophysics, 1973
- Reduced triphosphopyridine nucleotide binding sites of the fatty acid synthetase of chicken liverBiochemistry, 1970