Metabolism of DDE, DDD, and DDT in sheep

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Abstract
Sheep were dosed orally for 28 consecutive days with DDE, DDD, or DDT individually, or with a mixture of these three compounds. In a second similar trial DDE was administered either alone or simultaneously with DDD and/or DDT. The dose rate for each compound in all cases was 1 mg ppīsomer/kg body weight/day. All sheep were sampled for blood and omental fat during the dosing period and the following year. Maximum concentrations determined in fat were in the order DDE > DDD > DDT in the approximate ratios 10: 2: 1. Rates of elimination from fat when dosing was stopped were in the order DDD > DDT >DDE, the half-life being 4, 9, and 14 weeks respectively. In fat, DDE appeared as a metabolite of DDD and DDT, and DDD as a metalobite of DDT. In the first trial the simultaneous administration of DDD and DDT with DDE appeared to stimulate the subsequent elimination of DDE from storage in fat, but this effect was not reproduced in the second trial. There was a correlation between DDE in fat (mg/kg = x) and DDE in whole blood ( μg/kg = y) expressed by the regression equation. x = 1.8y-1.1 A sheep was given one oral dose of DDE, DDD, and DDT simultaneously, each at the rate of 50 mg/kg body weight. Highest concentrations of DDT and DDE occurred in blood 15 hours and 32 hours respectively after dosing. DDD showed two maxima, at 8 hours and at 32 hours after dosing. The value of blood and fat analyses for diagnostic purposes is discussed in relation to the results of these trials.

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