Assay of Chloramphenicol in Biological Media by High Performance Liquid Chromatography with U.V. Absorbance as the Detection Mode

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Abstract
Measurement of chloramphenicol in plasma, milk, urines and tissues was realized by high-performance liquid chromatography, with U.V. absorbance as the detection mode, at a wavalength set at 280 nm. Our method met requirements of sensitivity set up by the Ministries : 0.02 p.p.m. for meat and offal, and 0.01 p.p.m. for milk. The regression analysis, realized for each standard curve by expressing the chromatographic peak heights as a function of chloramphenicol levels in plasma, milk or urines, or chloramphenicol quantities in tissues were linear as far as our concentration range is concerned. The variation coefficients, determined by the repeatability assay on 10 samples at different concentrations were 1.37 to 13.29 ug/ml.