Genomic DNA Amplification from a Single Bacterium
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Open Access
- 1 June 2005
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 71 (6) , 3342-3347
- https://doi.org/10.1128/aem.71.6.3342-3347.2005
Abstract
Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using φ 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying nonculturable microorganisms.Keywords
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