Protein p53 Modulates Transcription from a Promoter Containing its Binding Site in a Concentration‐Dependent Manner
- 1 December 1995
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 234 (3) , 827-831
- https://doi.org/10.1111/j.1432-1033.1995.827_a.x
Abstract
Tumor suppressor protein p53 binds to DNA in a sequence-specific manner and activates transcription from promoters near its binding site. It is also known to repress promoters lacking the p53-binding site. In this study, we demonstrate that p53 can act as a transcriptional activator or repressor in vivo using the same reporter with the DNA-binding site CON and these effects depend on the amount of p53 expressed. Both in Saos2 and Cos7 cells, lower concentrations of p53 lead to activation and higher concentrations lead to repression of the model promoter containing the consensus p53-binding site CON. The N-terminal part of p53 is necessary for the transcriptional activation. It is not needed, however, for the repression of the same promoter, indicating that different domains of p53 are involved in activation and repression.Keywords
This publication has 39 references indexed in Scilit:
- Crystal Structure of a p53 Tumor Suppressor-DNA Complex: Understanding Tumorigenic MutationsScience, 1994
- Several hydrophobic amino acids in the p53 amino-terminal domain are required for transcriptional activation, binding to mdm-2 and the adenovirus 5 E1B 55-kD protein.Genes & Development, 1994
- p21 is a universal inhibitor of cyclin kinasesNature, 1993
- WAF1, a potential mediator of p53 tumor suppressionCell, 1993
- Specific repression of TATA-mediated but not initiator-mediated transcription by wild-type p53Nature, 1993
- Regulation of the Human hsp70 Promoter by p53Science, 1993
- Wild-type p53 mediates positive regulation of gene expression through a specific DNA sequence element.Genes & Development, 1992
- p53 Mutations in Human CancersScience, 1991
- Activation and repression of transcription by the gap proteins hunchback and Krüppel in cultured Drosophila cells.Genes & Development, 1991
- Mutations in the p53 gene occur in diverse human tumour typesNature, 1989