Detection of genetic diversity by pulsed-field gel electrophoresis among Escherichia coli O157 isolated from bovine faecal samples by immunomagnetic separation technique
- 1 January 2007
- journal article
- Published by Wiley in Letters in Applied Microbiology
- Vol. 44 (1) , 19-23
- https://doi.org/10.1111/j.1472-765x.2006.02034.x
Abstract
Escherichia coli O157 is considered to be one of most important human pathogens of animal origin which causes serious clinical complications. One of the most common methods to isolate E. coli O157 is the immunomagnetic separation (IMS) technique which employs specific antibodies coupled to magnetic beads to bind and extract cells from enrichment broths followed by plating onto sorbitol MacConkey agar supplemented with cefixime and potassium tellurite (CT-SMAC) plates. The aim of this study was to determine strain variation by pulsed-field gel electrophoresis (PFGE) among E. coli O157 on IMS/CT-SMAC plates. Every suspect colony of E. coli O157 was tested following isolation by the IMS/CT-SMAC technique. From 124 colonies detected; six XbaI-PFGE profiles were identified. Our results demonstrate that mixed populations of E. coli O157 with distinguishable PFGE profiles that are simultaneously present in bovine faeces can be isolated with IMS/CT-SMAC technique. If the aim of the study was to analyse diversity of PFGE profiles of E. coli O157 in a faecal sample following isolation by the IMS/CT-SMAC technique, at least five colonies per sample should be analysed to detect different PFGE subtypes if present.Keywords
This publication has 18 references indexed in Scilit:
- Comparison of Diversities ofEscherichia coliO157 Shed from a Cohort of Spring-Born Beef Calves at Pasture and in HousingApplied and Environmental Microbiology, 2005
- Distribution of Escherichia coli O157 in Bovine Fecal Pats and Its Impact on Estimates of the Prevalence of Fecal SheddingApplied and Environmental Microbiology, 2004
- High-Level Genotypic Variation and Antibiotic Sensitivity among Escherichia coli O157 Strains Isolated from Two Scottish Beef Cattle FarmsApplied and Environmental Microbiology, 2004
- Temporal Shedding Patterns and Virulence Factors ofEscherichia coliSerogroups O26, O103, O111, O145, and O157 in a Cohort of Beef Calves and Their DamsApplied and Environmental Microbiology, 2004
- Prevalence of Escherichia coli O157 in Cattle Feeds in Midwestern FeedlotsApplied and Environmental Microbiology, 2003
- Diversity, Frequency, and Persistence ofEscherichia coliO157 Strains from Range Cattle EnvironmentsApplied and Environmental Microbiology, 2003
- Within-Sample and Between-Sample Variation of Antimicrobial Resistance in FecalEscherichia coliIsolates from PigsMicrobial Drug Resistance, 2002
- The epidemiology, pathogenicity and microbiology of foodborne Escherichia coli O157:H7Reviews in Medical Microbiology, 2002
- Prevalence, Antibiotic Susceptibility, and Diversity of Escherichia coli O157:H7 Isolates from a Longitudinal Study of Beef Cattle FeedlotsApplied and Environmental Microbiology, 2001
- Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in JapanEpidemiology and Infection, 1999