Comparative properties of microsomes from cow's milk and from mammary gland. 2. Chemical composition
- 1 May 1958
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 69 (1) , 44-53
- https://doi.org/10.1042/bj0690044
Abstract
Several methods for estimation of the nucleic acid content of cytoplasmic particles from animal tissues were investigated. A procedure is described which gave satisfactory results on the basis of agreement between estimates based on absorption at 260 m[mu] and organic phosphorus content. The chemical composition of a preparation of disrupted sarcosomes from pig heart was determined. Microsomes from rat liver and from mammary gland of the lactating cow contained relatively high proportions of phospholipid and of nucleic acids, whereas microsomes from cow''s milk contained much less lipid and considerably less nucleic acid. Incubation of mammary-gland microsomes in milk serum caused some loss of lipid and considerable loss of nucleic acid, so that the product had a composition closely resembling that of microsomes from milk. The significance of these results in relation to the origin of milk microsomes is discussed.Keywords
This publication has 27 references indexed in Scilit:
- Comparative properties of microsomes from cow's milk and from mammary gland. 1. Enzymic activitiesBiochemical Journal, 1958
- The chemical composition of microsomes and mitochondria from silver beetBiochemical Journal, 1956
- Biochemistry of Cellular ParticlesAnnual Review of Biochemistry, 1956
- Alkaline phosphatase of milk. 2. Purification of the enzymeBiochemical Journal, 1953
- A Criticism of the Indiscriminate Use of the Schmidt-Thannhauser Method for the Fractionation of Nucleic Acids in Biological MaterialScience, 1953
- Microsomal Particles of Normal Cow's MilkNature, 1953
- Isolation of Hydrolytic Products of a Glycerophospho-compound from Micrococcus pyogenesJournal of General Microbiology, 1951
- Phosphorus compounds in the cell. 1. Protein-bound phosphorus fractions studied with the aid of radioactive phosphorusBiochemical Journal, 1951
- THE NUCLEIC ACIDS OF PLANT TISSUES .1. THE EXTRACTION AND ESTIMATION OF DESOXYPENTOSE NUCLEIC ACID AND PENTOSE NUCLEIC ACID1950
- Activity of the cytochrome system in heart muscle preparationsBiochemical Journal, 1947