Translation of Human Interleukin 2 mRNA inXenopus laevisOocytes
- 1 January 1983
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 27 (1) , 87-94
- https://doi.org/10.1111/j.1348-0421.1983.tb03570.x
Abstract
Poly(A)-positive mRNA extracted from tonsillar mononuclear cells stimulated with phytohemagglutinin-M and 12-o-tetradecanoyl phorbol 13-acetate was successfully translated into biologically active interleukin 2 (IL-2) in X. laevis oocytes, and secreted into the incubation medium. The extract of oocytes injected with either poly(A)-negative RNA or buffer did not show any IL-2 activity. By sucrose density gradient centrifugation analysis, IL-2 mRNA was found as a single peak corresponding to a sedimentation coefficient of 10-11S.This publication has 23 references indexed in Scilit:
- Kinetics of induction and molecular size of mRNAs encoding human interleukin-2 and γ-interferonNature, 1982
- The Biochemistry, Biology, and Role of Interleukin 2 in the Induction of Cytotoxic T Cell and Antibody‐Forming B Cell ResponsesImmunological Reviews, 1982
- Molecular Characterization of Interleukin 2Immunological Reviews, 1982
- Heterogeneity of human T-cell growth factor(s) due to variable glycosylationMolecular Immunology, 1981
- Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.The Journal of Experimental Medicine, 1980
- Concanavalin A-inducible, interleukin-2-producing T cell hybridoma.The Journal of Experimental Medicine, 1980
- T‐Cell Growth FactorImmunological Reviews, 1980
- Long term culture of tumour-specific cytotoxic T cellsNature, 1977
- Selective in Vitro Growth of T Lymphocytes from Normal Human Bone MarrowsScience, 1976
- Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living CellsNature, 1971