3′-processing of yeast tRNATrp precedes 5′-processing

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Abstract
Previous analyses of eukaryotic pre-tRNAs processing have reported that 5′-cleavage by RNase P precedes 3′-maturation. Here we report that in contrast to all other yeast tRNAs analyzed to date, tRNATrp undergoes 3′-maturation prior to 5′-cleavage. Despite its unusual processing pathway, pre-tRNATrp resembles other pre-tRNAs, showing dependence on the essential Lsm proteins for normal processing and efficient association with the yeast La homolog, Lhp1p. tRNATrp is also unusual in not requiring Lhp1p for 3′ processing and stability. However, other Lhp1p-independent tRNAs, tRNA2Lys and tRNA1Ile, follow the normal pathway of 5′-processing prior to 3-processing.