Analogy and homology of the dehydrogenases of Oomycetes. I. Regulation of glutamic dehydrogenases and isozyme patterns

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Abstract
The NAD-specific glutamic dehydrogenase isozymes of about 50 species of the Oomycetes were examined by polyacrylamide gel electrophoresis to determine their electrophoretic properties. With few exceptions, they had similar electrophoretic properties. Six allosteric modulators, NADP+, P-enolpyruvate, GTP, ATP, UTP, and short-chain acyl-CoA derivatives that stimulate the activity of the enzyme were used as a chemical parameter to distinguish the various isozymes. A taxonomic correlation was shown to exist at the level of enzyme control, for members of the Peronosporales (Pythium spp.) were more sensitive to allosteric controls than the Saprolegniales and Leptomitales. Among the Saprolegniales, Achlya spp. were the most sensitive. Responses by Aphanomyces spp., Isoachlya spp., Protoachlya spp., and Thraustotheca spp. were weak. The two species of the Leptomitales examined were completely insensitive to the allosteric modulators. Studies with citrate as an allosteric inhibitor was restricted to the Peronosporales for this study.

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