HPLC Laser Fluorometric Determination of Amines in Beer

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Abstract
A method has been developed for the rapid and accurate determination of the predominant aliphatic amines in beer. Chromatography was performed on a reverse phase C18 column using an acetonitrile-water solvent gradient. Chromatographic detection was facilitated by pre-column derivatization with 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) which fluoresces under visible light (in this case an argon ion laser operating at 488 nm) after reaction with an amine. Response was linear over four decades of concentration with detection limits at approximately five picograms injected.

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