4-Nitrobenzoic Acid Reductase of the NematodeAscaris lumbricoidesvarsuum. Localization of the Enzyme and Optimum Assay Conditions

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Abstract
1. Reduction of 4-nitrobenzoic acid by the nematode Ascaris lumbricoides var suum occurred in the intestinal brush border cells. Cuticle, mesenchyme fluid and reproductive tissue showed no activity.2. 4-Nitrobenzoic acid reductase was in the supernatant fraction after centrifugation of intestinal homogenates at 75000 g for 2 h.3. The enzyme required as cofactors NADH2, a low molecular weight thiol such as glutathione or cysteine and a divalent metal ion such as Mn++ or Cu++. The reduction was not catalysed by NADPH2.4. Reduction was not inhibited by O2 or CO. Optimum pH of the reaction was about 6·5.