Rhodanese from Thiobacillus A2: Determination of Activity by Proton Nuclear Magnetic Resonance Spectroscopy
- 1 December 1976
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 97 (2) , 285-288
- https://doi.org/10.1099/00221287-97-2-285
Abstract
Summary: Rhodanese from Thiobacillus A2 was shown by proton nuclear magnetic resonance (NMR) spectroscopy to use dihydrolipoate or dihydrolipoamide as acceptor of the sulphane moiety of thiosulphate with the formation of α-lipoate or lipoamide respectively. Correlation is shown between assays of the enzyme activity by NMR spectroscopy and by ultraviolet spectrophotometry.Keywords
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