Molecular forms of glucagon‐like peptides in man
Open Access
- 18 November 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 192 (2) , 275-278
- https://doi.org/10.1016/0014-5793(85)80124-1
Abstract
Molecular forms of the glucagon‐like peptides (GLP) encoded by the human preproglucagon gene were analysed by chromatography combined with specific radioimmunoassays to the synthetic peptides. Whereas extracts of human pancreas and a glucagonoma contained a large proglucagon cleavage product possessing both GLP‐1 and GLP‐2 immunoreactivities, extracts of human intestine contained products corresponding to free GLP‐1 and a small amount ofchromatographically distinct GLP‐2 immunoreactivity. It is concluded that post‐translational processing of proglucagon differs in pancreas and intestine, so that the C‐terminal portion of the molecule is cleaved to liberate free GLP‐1 in the intestine. Further processing or degradation results in loss especially of GLP‐2 immunoreactivity.Keywords
This publication has 7 references indexed in Scilit:
- Molecular Forms of Glucagon-Like Peptide-1 in Human Pancreas and GlucagonomasJournal of Clinical Endocrinology & Metabolism, 1985
- Glucagon gene sequence. Four of six exons encode separate functional domains of rat pre-proglucagon.Journal of Biological Chemistry, 1984
- Conversion of proglucagon in pancreatic alpha cells: the major endproducts are glucagon and a single peptide, the major proglucagon fragment, that contains two glucagon-like sequences.Proceedings of the National Academy of Sciences, 1984
- Molecular Forms of Human Enteroglucagon in Tissue and Plasma: Plasma Responses to Nutrient Stimuli in Health and in Disorders of the Upper Gastrointestinal Tract*Journal of Clinical Endocrinology & Metabolism, 1983
- Exon duplication and divergence in the human preproglucagon geneNature, 1983
- Molecular Forms of Glucagon-Like Immunoreactivity in Porcine Intestine and Pancreas*Endocrinology, 1983
- A theory of gel filtration and its exeperimental verificationJournal of Chromatography A, 1964