A method to culture immature embryos of Populusdeltoidesinvitro

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Abstract
Immature embryos were excised from capsules of Populusdeltoides Bartr. ex Marsh, resulting from the artificial pollination of female branches maintained in moist soil. Embryos excised 10–21 days postpollination produced multiple shoots (2–30) when cultured on a modified Murashige and Skoog medium. Embryos 28–35 days postpollination produced single or multiple shoots when cultured on the same medium. Embryos grown in reduced light produced more vigorous shoots than embryos grown at higher light intensities. The addition of glutamine to the medium in reduced light stimulated the embryos to thicken and increased the quality of the shoots produced. The methods described may be useful in rescuing embryos resulting from artificial hybridization in cases where they might otherwise be lost.

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