The isolation of β -histone from calf thymocytes and the factors affecting its aggregation

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Abstract
[beta]-Histone from the thymus gland of the calf has been prepared free from the other histone components by methods which have also been found to be applicable to the isolation of similar material from various tissues of the ox, chicken, rat and pig. Studies of the effect of physical conditions on the aggregation of ox thymus [beta]-histone have shown that this process occurs in two successive stages, which have been termed primary and secondary aggregation, respectively. By taking advantage of the discovery that both types of aggregation are repressed by polyvalent cations it has been possible to measure the sedimentation velocities of the unaggregated material, for which an S20 value of 2.0 x 10-13 has been obtained. This leads to a molecular weight of 57000 for unaggregated ox thymus B-histone. The primary aggregates of the [beta]-histone from the same source possess a molecular weight of 9 to 10 times this value. The amino-acid compositions of the pure [beta]-histones from the thymus gland and the liver of the ox have been determined.

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