USE OF LASER-NEPHELOMETER TO QUANTITATE LIPOPROTEIN-BOUND IMMUNOGLOBULINS IN HUMAN SERA - IMPORTANCE OF DILUTING REAGENT

  • 1 January 1978
    • journal article
    • letter
    • Vol. 29  (3) , 84-86
Abstract
If the method using Hyland LAS-R kit reagent recommended by the manufactorer yielded excellent results to measure immunoglobulins [Ig] free in serum, the same was not true when one quantitated Ig bound with antigens, especially with lipidic antigens. Since the specificity of Hyland antisera was not the cause of this difference, the effect of the diluting buffer on the antisera, on the blank and its consequences on the assay were studied. The abnormalities encountered in the results were due to precipitation of lipoproteins and immune complexes by the diluting buffer yielded in the kit. These errors were avoided by preparing a blank, not in NaCl 0.15 M, but in the kit reagent used to dilute the antisera.