Localization in diphtheria toxin fragment B of a region that induces pore formation in planar lipid bilayers at low pH
- 22 August 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 160 (1-2) , 82-86
- https://doi.org/10.1016/0014-5793(83)80941-7
Abstract
Like diphtheria toxin and the N-terminal (M r 23 000) region of fragment B, CB1 (M r 13 000), the cyanogen bromide peptide located in the middle region of fragment B is able to induce pore formation in lipid bilayer membrane at low pH. These two peptides (M r 23 000 and 13 000) share a common segment (M r 6300) containing the predicted amphipathic, α-helical, transverse lipid-associating domain (M r 2750) of fragment B[J. Cell Biol. (1980) 87, 837–840]. Therefore, we postulated this domain to be responsible for the pore formation ability of diphtheria toxin [Proc. Natl. Acad. Sci. USA (1981) 78, 172–176]. A relationship between the pH dependency of pore formation and the presence of a cluster of prolines in the C-terminal region of CB1 is proposed.Keywords
This publication has 20 references indexed in Scilit:
- Structure-activity relationships of the B fragment of diphtheria toxin: The lipid-binding domainsToxicon, 1982
- Diphtheria toxin fragment forms large pores in phospholipid bilayer membranes.Proceedings of the National Academy of Sciences, 1981
- A CNBR peptide located in the middle region of diphtheria toxin fragment B induces conductance change in lipid bilayersBiochemical and Biophysical Research Communications, 1981
- Diphtheria toxin forms transmembrane channels in planar lipid bilayers.Proceedings of the National Academy of Sciences, 1981
- The entry of diphtheria toxin into the mammalian cell cytoplasm: evidence for lysosomal involvement.The Journal of cell biology, 1980
- Primary structure of diphtheria toxin fragment B: structural similarities with lipid-binding domains.The Journal of cell biology, 1980
- Diphtheria toxin entry into cells is facilitated by low pH.The Journal of cell biology, 1980
- Diphtheria ToxinAnnual Review of Biochemistry, 1977
- Binding of triton X-100 to diphtheria toxin, crossreacting material 45, and their fragments.Proceedings of the National Academy of Sciences, 1976
- Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfateAnalytical Biochemistry, 1971