Peroxynitrite formed by simultaneous generation of nitric oxide and superoxide selectively inhibits bovine aortic prostacyclin synthase
- 11 March 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 382 (1-2) , 101-104
- https://doi.org/10.1016/0014-5793(96)00160-3
Abstract
The effect of various oxidants on bovine aortic prostacyclin synthase was tested with 14C-labelled prostaglandia endoperoxide as substrate. No sensitivity against hydrogen peroxide, superoxide or hydroxyl radicals was observed but hypochlorite inhibited with an IC50 value of 7 μM. Among the reactive nitrogen species nitric oxide and nitrogen dioxide radicals were ineffective, but peroxynitrite irreversibly blocked prostacyclin biosynthesis with an IC50 value of 50 nM. Peroxynitrite acted within seconds whereas hypochlorite required up to 30 min for completion. Simultaneous generation of nitric oxide and superoxide also caused inhibition which suggested that under pathological conditions like ischemia-reperfusion not only the vasodilatory effects of nitric oxide but also those of prostacyclin could be eliminated.Keywords
This publication has 16 references indexed in Scilit:
- The role of nitric oxide and other endothelium-derived vasoactive substances in vascular diseaseProgress in Cardiovascular Diseases, 1995
- Sensitivity of the Essential Zinc-Thiolate Moiety of Yeast Alcohol Dehydrogenase to Hypochlorite and PeroxynitriteBiochemistry, 1995
- Mechanism of covalent modification of glyceraldehyde‐3‐phosphate dehydrogenase at its active site thiol by nitric oxide, peroxynitrite and related nitrosating agentsFEBS Letters, 1994
- Tumor necrosis factor enhances low density lipoprotein oxidative modification by monocytes and endothelial cellsFEBS Letters, 1994
- Pharmacology of the Endothelium in Ischemia-Reperfusion and Circulatory ShockAnnual Review of Pharmacology and Toxicology, 1993
- Modulation of Leukocyte-Mediated Myocardial Reperfusion InjuryAnnual Review of Physiology, 1990
- Reaction of No With O2−. Implications for the Action of Endothelium-Derived Relaxing Factor (EDRF)Free Radical Research Communications, 1990
- Oxidant-induced DNA damage of target cells.Journal of Clinical Investigation, 1988
- Preparative hplc purification of prostaglandin endoperoxides and isolation of novel cyclooxygenase-derived arachidonic acid metabolitesBiochemical Pharmacology, 1987
- Chemical synthesis with a quenched flow reactor. Hydroxytrihydroborate and peroxynitriteJournal of the American Chemical Society, 1974