The relation between the anterior pituitary body and the gonads.―Part. III.―Fractionation and dilution of ovary-stimulating extracts

Publisher Website
Google Scholar
Abstract
To determine whether the two ovarian reactions follicular luteinization and formation of atretic corpora lutea without ovulation, and normal follicular maturation and ovulation, were to be referred to one or more than one stimulating hormone, fractionation and dilution of ovary-stimulating extracts were undertaken. Extensive dilution experiments failed to indicate that different concentrations of a single hormone might account for both effects. On the other hand, the view that 2 hormones might be concerned was supported by the effects of alcoholic fractions of urine extracts. Solutions of the latter in 50% alcohol were predominantly luteinizing, solutions in 30% alcohol tending rather to promote follicular maturation. These findings, unexplained on a quantitative basis, appeared to indicate a separation of different stimulating substances by the process of fractionation.