MICROPHOTOMETRICAL QUANTIFICATION OF PROTEIN THIOLS IN MORPHOLOGICALLY INTACT-CELLS OF CERVICAL EPITHELIUM .1. BASIC RESULTS WITH SUPERFICIAL CELLS OF HEALTHY WOMEN

  • 1 January 1977
    • journal article
    • research article
    • Vol. 21  (2) , 341-344
Abstract
The investigations are based on Esterbauer''s results according to which the staining of protein thiols (PSH) with DDD-Fast Blue B (Barrnett and Seligman) is of a quantitative order. The PSH of the nucleus and the cytoplasm of superficial cells of the ectocervical epithelium of healthy women was 0.3 and 4.1 .times. 10-14 mol, respectively. After it was found that the mean absorption spectrum between 310 and 238 nm of the cytoplasm of 90 cells corresponded to that of pure proteins, the extinction at 278 nm of a cytoplasmic area (300-400 .mu.2) of unstained cells was taken as a 1st measure for the cytoplasmic protein content which was calculated to approximately 5 .times. 10-18 mol/.mu.2; the PSH content was 1.9 .times. 10-17 mol/.mu.2. A plausible number of 3.6 SH/105 g of cytoplasmic proteins results from this value.

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