Synthese der alkalischen Phosphatase in einem stringent und relaxed kontrollierten Stamm von Escherichia coli nach Aminosäuren‐ und Phosphatlimitation

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Abstract
Synthesis of alkaline phosphatase in Escherichia coli is derepressed under phosphate starvation in the stringent strain CP78 as well as in its relaxed counterpart CP79. During limitation of phosphate as well as of amino acids a decrease of enzyme activity is observed, especially in the relaxed strain, which can not produce ppGpp under this conditions.After phosphate limitation synthesis of ppGpp is not stimulated and the kinetics of RNA synthesis is similar in both strains.We suggest that ppGpp is not directly involved in the regulation of gene expression during phosphate starvation.