Analysis of Organ Specificity of a Low Temperature Responsive Gene Family in Rye (Secale cerealeL.)

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Abstract
The barley (Hordeum vulgare L.) low temperature responsive gene blt14 was used as a probe, to isolate two different cognate clones (rlt1412; rlt1421) from a rye (Secale cereale L.) cDNA library prepared from low temperature-treated (6°C day/2 °C night) shoot meristems of the cultivar, Puma. Northern blot analysis revealed that low temperature expression of rlt1412 is highest in root tissues whereas, rlt1421 shows greatest mRNA accumulation in mature leaf tissues. There is a relationship between the steady-state levels of these mRNA species and the frost hardiness of Puma (North American cultivar) and Rhayader (UK cultivar) such that the expression ofboth genes is higher in the more frost hardy cultivar, Puma, compared with Rhayader. DNA and predicted amino acid sequence analysis indicated that the rye and barley clones encode small proteins with consensus N-terminal signal sequences whose biological function is at present unknown. The relevant sequences are lodged in the EMBL data base.

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