Enzymatic biosynthesis of cephalexin
- 1 June 1980
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 22 (6) , 1237-1247
- https://doi.org/10.1002/bit.260220610
Abstract
The reaction kinetics of the enzymatic of cephalexin from 7‐aminodea‐cetoxy cephalosporanic acid and phenylglycine methylester was studied using the synthesizing enzyme obtained from Xanthomonas citri. The activation energy, Km value for 7‐aminodeacetoxy cephalosporanic acid and phenylglycine methylester, and Ki value for phenylglycine methylester were determined as 8.63 kcal/mol, 3.7mM, 14.5mM, and 70mM, respectively. The enzyme was found to be constitutive and susceptible to deactivation.This publication has 15 references indexed in Scilit:
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