USE OF NEUTRAL RED FLUORESCENCE FOR THE IDENTIFICATION OF COLONIES OF CLOSTRIDIA

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Abstract
Inoculated liver veal agar (Difco) to which 0.004% neutral red had been added was poured into plates between base and cap layers of the same medium. The plates were incubated in a hydrogen atmosphere for 18 to 24 hr. Colonies of all the strains of clostridia that were studied produced zones of golden yellow fluorescence that were visible with UV after 18 to 24 hr. and with ordinary illumination after 24 to 48 hr. No facultative organisms and only 2 of numerous strains of obligate anaerobic nonsporeformers produced the fluorescence. The procedure was used to speed the isolation of clostridia from various sources. Only colonies of clostridia produced the fluorescence and cultures could be obtained from the primary colonies. The fluorescence produced by the clostridia differed from that produced by reduction with Zn in that it was more rapid and was less affected by the pH of the medium.

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