Activity of DNase associated with replication of Epstein-Barr virus in NPC-204 cells.

  • 1 August 1984
    • journal article
    • Vol. 17  (3) , 121-30
Abstract
A deoxyribonuclease derived from Epstein-Barr virus (EBV)-producing lymphoblastoid cell line (NPC-204 cells) treated with IUdR was purified by DEAE cellulose, phospho-cellulose, and DNA-cellulose columns with 45% recovery. The activity of the DNase was neutralized by serum of patient with nasopharyngeal carcinoma (NPC). The DNase had a strong requirement for divalent cations and an alkaline pH optimum. Its activity was inhibited by highly ionic strength and polyamines.

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