Destruction of Hamster Ovarian Cell Cultures by Peritoneal Macrophages From Mice Treated With Corynebacterium granulosum

Abstract
We studied the effect of peritoneal macrophages from C3Hf/Bu mice treated with Corynebacterium gtanulosum on the in vitro growth of a continuously cultured line of Chinese hamster ovary (CHO) cells. Growth of these cells was greatly inhibited when plated on C. granulosum-stimulated macrophages in ratios of 1:34, 1:68, and 1:136, with a greater effect as the ratio increased. The colony-forming ability of target CHO cells was completely inhibited by the presence of stimulated macro phages at a ratio of 1:136. At this ratio of CHO cells to normal macrophages, however, the ability of CHO cells to form colonies was only slightly reduced. Neither normal nor C. granulosum- stimulated macrophages affected the in vitro growth of syngeneic fibroblasts. The results, therefore, indicated that C. granufosum activated macrophages to destroy a xenogeneic cell line in an immunologically nonspecific manner, with no cytotoxic effect on a normal syngeneic cell strain.

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