Complex Binding of the Embryonic Interferon, Ovine Trophoblast Protein-1, to Endometrial Receptors

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Abstract
Ovine embryos produce an interferon (IFN)-αII in significant quantities during early pregnancy. This IFN, previously termed ovine trophoblast protein-1 (oTP-1), is a 172-amino-acid polypeptide which has been suggested to be the causal agent in maternal recognition of pregnancy in the ewe. Here we report the binding of oTP-1 and a recombinant bovine IFN-αI1 (rBoIFN-αI1; 165–166 amino acids long) to membrane preparations from ovine uterine endometrium. Both oTP-1 and rBoIFN-αI1 competed with each other for receptor binding. Based on Scatchard analysis, [125I]oTP-1 binding was determined to be complex and resolvable into a high-affinity (Kd = 3.8 × 10−11M, 30 fmoles/mg protein) and a low affinity (Kd = 1.7 × 10−10M; 96 fmoles/mg protein) component. Conversely [125I]rBoIFN-αI1 bound to only a single high-affinity receptor (Kd = 6.1 × 10−11M; 174 fmoles/mg protein). Cross-linking experiments using disuccinimidyl suberate revealed that [125I]oTP-1 associated with membrane polypeptides of two molecular weight classes (Mr 100,000 and 70,000), and could be displaced from both with rBoIFN-αI1. In contrast, [125I]rBoIFN-αI1 cross-linked to only the 100,000 Mr membrane polypeptide. These data provide evidence that the binding parameters of oTP-1 and rBoIFN-αI1 to endometrial receptors are different.