Increased Rate of DNA Recovery from United Kingdom Epidemic Clostridium difficile PCR Ribotype 1 Strains Stored Cryogenically
- 1 November 2005
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 43 (11) , 5794-5
- https://doi.org/10.1128/jcm.43.11.5794-5795.2005
Abstract
We noted that some Clostridium difficile isolates are nonrecoverable after frozen storage and so used molecular typing analysis to characterize DNA from these strains. The recovery rate of C. difficile PCR ribotype 1 was statistically significantly greater than that of other strains. This observation has implications for C. difficile epidemiological studies.Keywords
This publication has 9 references indexed in Scilit:
- Evidence to support the existence of subgroups within the UK epidemic Clostridium difficile strain (PCR ribotype 1)Journal of Hospital Infection, 2003
- The effects of storage conditions on viability of Clostridium difficile vegetative cells and spores and toxin activity in human faecesJournal of Clinical Pathology, 2003
- Typing of Clostridium difficileClinical Microbiology & Infection, 2001
- Molecular epidemiology of endemic Clostridium difficile infectionEpidemiology and Infection, 2001
- Hospital disinfectants and spore formation by Clostridium difficileThe Lancet, 2000
- Value of lysozyme agar incorporation and alkaline thioglycollate exposure for the environmental recovery ofClostridium difficileJournal of Hospital Infection, 2000
- Epidemics of Diarrhea Caused by a Clindamycin-Resistant Strain ofClostridium difficilein Four HospitalsNew England Journal of Medicine, 1999
- Factors associated with prolonged symptoms and severe disease due to Clostridium difficileAge and Ageing, 1999
- A Simple Method for Storage of Bacteria at — 76°CJournal of Applied Bacteriology, 1978