LONG-TERM PERFUSION OF HUMAN CORNEAS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 18  (1) , 107-111
Abstract
In 12 isolated human corneas (cadaver time 2-16 h) stromal thickness could be maintained within 0.50-0.66 mm up to 20 h by the use of an endothelial bathing medium consisting of glutathione-bicarbonate Ringer''s solution, HEPES-buffer and 20% human serum. During the first 3-5 h of perfusion, temperature reversal occurred in all corneas. Wound healing processes were observed in 9 corneas throughout the experiments after scraping off a 2.5 mm area of central epithelium, which was kept in a moist saline-saturated atmosphere.

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