Induction of Mycobacterium avium proteins upon infection of human macrophages

7 October 2004

journal article
research article
Published by Wiley in Proteomics

Vol. 4 (10) , 3078-3083
https://doi.org/10.1002/pmic.200300891

Abstract

Induction of Mycobacterium avium proteins labelled with [³⁵S]methionine and mRNAs upon infection of the human macrophage cell line THP-1 was investigated by two-dimensional gel electrophoresis-mass spectrometry and reverse transcriptase-polymerase chain reaction (RT-PCR), respectively. M. avium overexpressed proteins within the macrophages that are involved in fatty acids metabolism (FadE2, FixA), cell wall synthesis (KasA), and protein synthesis (EF-tu). The correlation of differential protein and mRNA expression varied between good and no correlation. Overall, these four proteins may be involved in the adaptation and survival of M. avium within human macrophages.

Keywords

This publication has 29 references indexed in Scilit:

Comparative proteome analysis of culture supernatant proteins from virulent Mycobacterium tuberculosis H37Rv and attenuated M. bovis BCG Copenhagen
Electrophoresis, 2003
Transcriptional Adaptation of Mycobacterium tuberculosis within Macrophages
The Journal of Experimental Medicine, 2003
Mycobacterium's Arrest of Phagosome Maturation in Macrophages Requires Rab5 Activity and Accessibility to Iron
Molecular Biology of the Cell, 2003
Mycobacterium aviumGenes Expressed during Growth in Human Macrophages Detected by Selective Capture of Transcribed Sequences (SCOTS)
Infection and Immunity, 2002
Human Macrophage Gamma Interferon Decreases Gene Expression but Not Replication ofMycobacterium tuberculosis: Analysis of the Host-Pathogen Reciprocal Influence on Transcription in a Comparison of Strains H37Rv and CMT97
Infection and Immunity, 2001
The Mycobacterium tuberculosis ECF sigma factor σ^E: role in global gene expression and survival in macrophages^†
Molecular Microbiology, 2001
A comparison of selected mRNA and protein abundances in human liver
Electrophoresis, 1997
Lack of Acidification in Mycobacterium Phagosomes Produced by Exclusion of the Vesicular Proton-ATPase
Science, 1994
The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences
Electrophoresis, 1993
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Proceedings of the National Academy of Sciences, 1979