Complex Fucolipids of Hog Gastric Mucosa Structure of the Ceramide Eikosahexoside

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Abstract
The structure of a complex fucolipid from hog gastric mucosa containing twenty sugar residues and exhibiting blood-group (A+H) activity has been investigated. Based on the results of immunological assays, partial acid hydrolysis, sequential degradation with specific exoglycosidases, oxidation with periodate and chromium trioxide, and permethylation analysis, we suggest that the carbohydrate chain of this fucolipid contains four termini. One of the termini bears βGal1→ 4βGlcNAc disaccharide, two bear blood-group A determinant and one bears H determinant. Two of the branches, terminated by βGal1 → 4βGlcNAc and blood-group A determinant, and two terminated by blood-group A and H determinants, are linked through βGal1→ 4βGlcNAc1 → 3/6 and βGal1 → 4βGlcNAc1→ 4βGlcNAc1→ 3/6 to the galactose residue adjacent to glucosylceramide core.