Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles
Top Cited Papers
- 20 September 2004
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 101 (39) , 14036-14039
- https://doi.org/10.1073/pnas.0406115101
Abstract
We find that single- and double-stranded oligonucleotides have different propensities to adsorb on gold nanoparticles in colloidal solution. We use this observation to design a hybridization assay based on color changes associated with gold aggregation. Because the underlying adsorption mechanism is electrostatic, no covalent functionalization of the gold, the probe, or the target DNA is required. Hybridization conditions can be optimized because it is completely separated from the detection step. The assay is complete within 5 min, and <100 femtomoles of target produces color changes observable without instrumentation. Single-base-pair mismatches are easily detected.Keywords
This publication has 23 references indexed in Scilit:
- Label-Free Colorimetric Detection of Specific Sequences in Genomic DNA Amplified by the Polymerase Chain ReactionJournal of the American Chemical Society, 2004
- Rapid Aggregation of Gold Nanoparticles Induced by Non-Cross-Linking DNA HybridizationJournal of the American Chemical Society, 2003
- Identification of a Novel Coronavirus in Patients with Severe Acute Respiratory SyndromeNew England Journal of Medicine, 2003
- Hybridization-Based Unquenching of DNA Hairpins on Au Surfaces: Prototypical “Molecular Beacon” BiosensorsJournal of the American Chemical Society, 2003
- Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA DetectionScience, 2002
- Shape and size dependence of radiative, non-radiative and photothermal properties of gold nanocrystalsInternational Reviews in Physical Chemistry, 2000
- A Multistep Chemical Modification Procedure To Create DNA Arrays on Gold Surfaces for the Study of Protein−DNA Interactions with Surface Plasmon Resonance ImagingJournal of the American Chemical Society, 1999
- Preparation and Characterization of Au Colloid MonolayersAnalytical Chemistry, 1995
- Functional group substitutions as probes of hydrogen bonding between GA mismatches in RNA internal loopsJournal of the American Chemical Society, 1991
- Photothermal deflection spectroscopy and detectionApplied Optics, 1981