Activity Standardization of Technical Preparations of Douglas-fir Tussock Moth Baculovirus123

Abstract
Polyhedron counts are the most commonly used “measure” of the potency of technical nucleopolyhedrosis virus preparations. The actual potency of such preparations, however, can be determined only by bioassay. If the bioassay is standardized, it provides a frame of reference for comparisons among microbial products and among results of field applications. We describe an activity standardization procedure based on the stability of the response of an inbred strain of Orgyia pseudotsugata (McDunnough). The response of strain CL-1 is the reference standard currently accepted by the U.S. Environmental Protection Agency and by the U.S. Forest Service for activity titrations of industrial preparations of Douglas-fir tussock moth Baculovirus. Tests covering a 2yr span and 3 generations of GL-I larvae show that the procedure is highly reproducible.

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