Concanavalin A-Induced Agglutination of Naegleria
- 1 September 1977
- journal article
- research article
- Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene
- Vol. 26 (5) , 856-858
- https://doi.org/10.4269/ajtmh.1977.26.856
Abstract
Concanavalin A (Con A) agglutinated all Naegleria gruberi strains tested but did not agglutinate any N. fowleri strains tested. Agglutination was time and temperature dependent and Con A concentration and ameba concentration dependent over certain ranges. Agglutination increased to maximum up to 1 h incubation with Con A. At least 1 × 106 amebae/ml were needed for maximum agglutination, and Con A concentrations higher than 100 µg/ml did not appreciably increase agglutination. Incubation at 4°C or with 10 mM α-methyl-D-mannoside inhibited agglutination of N. gruberi. These data indicate a difference in polysaccharide structure of cell membranes of N. fowleri and N. gruberi.This publication has 1 reference indexed in Scilit:
- Primary Amoebic Meningoencephalitis Caused by NaegleriaAnnals of Internal Medicine, 1971