Kinetics of desynchronization and distribution of generation times in synchronized cell populations.
- 1 September 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (9) , 4404-4407
- https://doi.org/10.1073/pnas.75.9.4404
Abstract
The kinetics of the distribution of generation times in synchronized [African green monkey CV-1 kidney] cells can be analyzed by using Fourier transform analysis. Deviations of the experimental data from the curve of completely asynchronous growth reflect the degree of synchrony at a particular time. Fourier transform analysis of these deviations yields the average generation time and as information on the distribution of generation times characterizing a synchronized culture. A detailed analysis of synchronized cell cultures does not provide any evidence for the existence of a subcycle or a polymodal distribution in generation times. Cell-cell interaction occurs at cell densities as low as 2.5 .times. 103/cm2. The Eyring-Stover formalism for the dynamics of survival can correctly describe the distribution of the first round of cell divisions in synchronized culture.This publication has 12 references indexed in Scilit:
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