Integration Site of Noninducible Coliphage 186

Abstract
From conjugational data, the attachment site for noninducible coliphage 186 ( att 186) was located between the origins of Hfr strains KL16 and KL98, and close to the pheA gene in Escherichia coli K-12. P1 transductions indicated that att 186 lies at 51 min on the standard genetic map of E. coli , with the order cysC-nalB-att 186- pheA . The presence of prophage 186 in the donor destroyed linkage between nalB and pheA , which is taken as evidence for the integration of the 186 prophage between these genes.