Gene Expression Enabling Synthetic Diversification of Natural Products: Chemogenetic Generation of Pacidamycin Analogs
- 16 August 2010
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 132 (35) , 12243-12245
- https://doi.org/10.1021/ja1060406
Abstract
Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.Keywords
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