SbvI restriction endonuclease from Streptococcus bovis
- 1 December 1993
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 17 (6) , 297-299
- https://doi.org/10.1111/j.1472-765x.1993.tb01471.x
Abstract
Restriction endonuclease SbvI, an isoschizomer of HaeIII, has been isolated from rumen amylolytic bacterium Streptococcus bovis II/1. SbvI was purified from cell extract by phosphocellulose chromatography and heparin-Sepharose chromatography. The recognition sequence of SbvI was identified by digestion of pBR322, pUC9 and lambda-DNA and comparing the cleavage patterns obtained with computer-derived data. SbvI recognizes the 4-bp palindrome, 5'-GGCC-3' and cleaves DNA after the second G in the sequence, producing blunt ends.Keywords
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