The p120 catenin partner Kaiso is a DNA methylation-dependent transcriptional repressor
Open Access
- 1 July 2001
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 15 (13) , 1613-1618
- https://doi.org/10.1101/gad.198501
Abstract
We describe a novel mammalian DNA binding activity that requires at least two symmetrically methylated CpG dinucleotides in its recognition sequence, preferably within the sequence 5′CGCG. A key component of the activity is Kaiso, a protein with POZ and zinc-finger domains that is known to associate with p120 catenin. We find that Kaiso behaves as a methylation-dependent transcriptional repressor in transient transfection assays. Kaiso is a constituent of one of two methyl–CpG binding complexes originally designated as MeCP1. The data suggest that zinc-finger motifs are responsible for DNA binding, and may therefore target repression to specific methylated regions of the genome. As Kaiso associates with p120 catenin, Kaiso may link events at the cell surface with DNA methylation-dependent gene silencing.Keywords
This publication has 32 references indexed in Scilit:
- Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse developmentGenes & Development, 2001
- DNA Recognition by the Methyl-CpG Binding Domain of MeCP2Published by Elsevier ,2001
- Cytosine methylation transforms an E2F site in the retinoblastoma gene promoter into a binding site for the general repressor methylcytosine- binding protein 2 (MeCP2)Nucleic Acids Research, 1999
- The BCL-6 POZ domain and other POZ domains interact with the co-repressors N-CoR and SMRTOncogene, 1998
- Inactivation of the E-Cadherin-Mediated Cell Adhesion System in Human CancersThe American Journal of Pathology, 1998
- A minisatellite “core” element constitutes a novel, chromatin-specific activator of mts1 gene transcriptionJournal of Molecular Biology, 1998
- Recognition of DMA methylation by zinc fingersNature Structural & Molecular Biology, 1998
- A Methylation-Dependent DNA-Binding Activity Recognising the Methylated Promoter Region of the MouseXistGeneBiochemical and Biophysical Research Communications, 1997
- DNA methylation inhibits transcription indirectly via a methyl-CpG binding proteinPublished by Elsevier ,1991
- Identification of a mammalian protein that binds specifically to DNA containing methylated CpGsPublished by Elsevier ,1989