Embryonic Cell Aggregation: Kinetics and Specificity of Binding of Enhancing Factors
- 1 March 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (3) , 727-731
- https://doi.org/10.1073/pnas.71.3.727
Abstract
A new assay for tissue-type-specific adhesive macromolecules is described. This assay measures the binding to whole cells of radioactive macromolecules in aggregation-enhancing supernatants. We show for two tissue types, embryonic chick neural retina and cerebrum, that binding is tissue-type specific. Specific binding can be distinguished from nonspecific binding by its sensitivity to displacement with horse serum and by the kinetics of binding. Specific binding appears to be a cooperative phenomenon.Keywords
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