Crystalline Leghemoglobin. 1. Purification Procedure.

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Abstract
A procedure is described for the purification of leghemoglobin to give the two main components in a crystalline state. The different components were isolated by chromatog-raphy using diethylaminoethyl cellulose as adsorbent. The main components were crystallised from slightly acid ammonium sulphate solution. Both of the crystalline components were found to be electro-phoretically homogeneous in long-time electrophoresis runs at two different pH values. The electrophoretically faster component contained 0.32% iron, and the slower one 0.29% of iron.

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