PARASITIZATION IN VITRO OF BOVINE CELLS BY BRUCELLA ABORTUS

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Abstract
Normal bovine fixed-tissue cells have been routinely grown in vitro as a monolayer on glass. Brucella abortus has been shown to invade, and to multiply rapidly within, bovine cells from fetal skin and kidney as well as adult uterine mucosa, testes, spleen, bone marrow, and lung. Similar intracytoplasmic growth patterns were observed in cells from various tissues. It has been found that B. abortus Type 1 multiplied to the same extent in cells from fetal skin and"adult uterine mucosa. B. abortus Type I and Type n, both CO2-dependent and CO2-indenpendent strains, invaded and multiplied within fetal skin cells at approximately the same rate. The number of intracellular brucellae depended, within limits, both on the number of extracellular organisms to which the cells were exposed and on the exposure time. Intracellular growth rates proved independent of these variables, at least during the first 72 hours of intracellular residence.