Fluid reabsorption in the isolated duct of the rat cauda epididymidis

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Abstract
A technique is described for measurement of rate of fluid reabsorption in a segment of the rat cauda epididymidis in vitro. The basal rate was 2.63 .+-. 0.22 .mu.l/cm2 per 30 min and was dependent on temperature and inhibited by 2,4-dinitrophenol, suggesting that fluid reabsorption is an energy-dependent process. About 50% of the fluid reabsorption was dependent on intraluminal Na ions. This Na+-dependent component was inhibited by addition of amiloride to the intraluminal fluid and ouabain to the peritubular fluid.